Glucosamine is a natural amino monosaccharide, which is not only a constituent mechanism of joint cartilage, but also widely distributed in connective tissue, skin tissue, and synovial fluid. Chondroitin sulfate is the main representative of medically proven viscosity-rich substances. Chondroitin sulfate can effectively replenish cartilage element proteoglycans, absorb and retain water in cartilage, lubricate joints, remove inflammatory substances from joint cavities, repair joint cartilage, and reduce joint pain, swelling, and stiffness. The combination of glucosamine and chondroitin sulfate can enhance the inhibition of the production of various inflammatory mediators and oxygen free radicals in joint tissues, inhibit metalloproteinase activity and stabilize lysosomal membranes, thus playing an anti-inflammatory and analgesic role. The combination of the two can also promote the synthesis of proteoglycans and collagen in articular cartilage tissue, maintain the stability of the extracellular matrix of cartilage, and also indirectly play a role in clearing inflammation and relieving pain. Clinical observation also shows that for the treatment of moderate to severe patients, the effect of the combination of the two is higher than that of a single drug, which can more effectively reduce the pain of patients.