Pre-conception check-ups are also needed for the second child, as people are living under more and more pressure at work or in life, and there are many possible causes of infertility. The woman should have a comprehensive health checkup: for example, height, weight, blood pressure, thyroid function, blood sugar, blood lipids, liver and kidney function, biochemical and eugenics tests, gynecological examination, ultrasound examination of uterine adnexa, and cervical cancer screening, and follicle monitoring test to guide intercourse if the woman has irregular menstruation. Pregnant women over 35 years of age need an endocrine test to assess ovarian reserve function. The male partner also needs a comprehensive health checkup: blood pressure, weight, height, blood sugar, lipids, thyroid, liver and kidney functions, eugenics, semen analysis, etc. If necessary, a tubal imaging and hysteroscopy will be done.