Hormonal drugs such as progesterone and estrogen cannot be used before checking basal sex hormones, except for those who need to be rechecked after treatment, of course. Sex hormones can be tested at different times of menstruation depending on the disease. For example, for the determination of basal levels, the 2nd to 5th day of menstruation should be selected for examination, such as progesterone determination should be checked during the luteal phase (21 days of menstruation or 7 days after ovulation); patients with irregular vaginal bleeding, such as testing gonads, should be checked for 6 items to prevent misdiagnosis of menstrual scarcity and amenorrhea.