Creatine kinase is found in a wide range of muscle tissues, including cardiac and skeletal muscle. Creatine kinase isoenzyme is an isoform of creatine kinase found in the heart, which, if elevated, usually indicates the presence of myocardial damage and is a specific marker for myocardial injury. Creatine kinase isozymes usually do not normalize on their own and require active treatment of the disease. Creatine kinase isoenzyme is normally undetectable or present in trace amounts in the blood. When diseases such as acute myocardial infarction and myocarditis occur, creatine kinase isoenzyme may become elevated. Elevated creatine kinase isoenzyme suggests the presence of myocardial injury, which should be examined thoroughly, the cause of the disease clarified as soon as possible, and treated aggressively. Creatine kinase isoenzyme can only decrease gradually after the damaged myocardium has recovered. Without treatment, its value will not return to normal on its own and should not be delayed. It is recommended to consult a doctor in time, improve the examination and standardize the treatment under the doctor’s guidance.