Antibiotics can affect the syphilis window period to some extent. Using antibiotics during the window period of syphilis is likely to prolong the window period and lead to false-negative syphilis antibody test, therefore, if antibiotics are used during the window period, it is important to appropriately prolong the time to do the syphilis antibody test. Syphilis is a sexually transmitted disease infected by the syphilis spirochete, and the main mode of transmission is sexual transmission, vertical transmission from mother to child, and blood transmission, etc. It manifests itself in the form of hard chancre (small erythematous spots on the external genitalia, ulcers, dark red scars, etc.), syphilis rash (brown papules, maculopapules, pustules, etc.), etc. It is mainly treated with penicillin, and if the penicillin is not tolerated it can be treated with ceftriaxone instead. There is a window period before the onset of syphilis, and if antibiotics are used during this period, it will prolong the window period, affecting the results of the syphilis antibody test, and false negatives may occur. It is recommended that patients go to the dermatology or infection department of regular hospitals in a timely manner, follow the doctor’s instructions, reasonable treatment, and when using medication, strictly comply with the doctor’s instructions to use medication, do not blindly self-medication. If antibiotics are used during the window, the time for syphilis antibody testing should be prolonged to avoid false negatives. During the period of syphilis, avoid sexual intercourse and sharing personal belongings with others.