Urinary tract infection is an inflammation of the urinary tract caused by the multiplication of a large number of bacteria and other microorganisms in the urinary tract, which may or may not have clinical symptoms such as urinary frequency, urinary urgency, and painful urination, and in severe cases may cause chills, high fever, or even life-threatening sepsis, and is a common and frequent disease, and many patients have serious health and quality of life problems due to recurrent urinary tract infections. Urine culture is the bacteriological culture of urine to identify the bacteria causing urinary tract infections and to clarify which antibacterial drugs are effective against the bacteria through drug sensitivity testing. Based on the results of the urine culture and the patient’s age, drug allergy, liver and kidney function, the doctor selects the appropriate drug, the appropriate dosage and medication method for treatment to enable the patient to recover as soon as possible. Many doctors and patients ignore the importance of urine culture and do not know what the causative bacteria are in different types of patients and what antibacterial drugs they are sensitive to, and often use drugs based on “experience”, resulting in unsatisfactory treatment, increased patient suffering, and even threatening patients’ lives due to delayed treatment. Therefore, it is recommended that doctors and patients do not forget to do urine culture before treatment of urinary tract infections.