HIV is clinically used as a test for HIV, and pregnant women are prone to false positive HIV tests during pregnancy. When a preliminary test is performed during pregnancy and a false-positive HIV report occurs, it is important to first clarify whether the reagents used to check HIV are in their expiration date and qualified to prevent any impact on the experimental results, and also in HIV infection testing is susceptible to various factors such as management system, operators and testing equipment, which cannot guarantee 100% accuracy and needs to be verified by rechecking. Many indicators of pregnant women’s bodies are not within the normal range during pregnancy, and HIV tests are prone to false positives. It is recommended that pregnant women undergo nucleic acid testing under the guidance of a doctor to determine the presence of HIV in their bodies. At the same time, it is important for pregnant women to take appropriate tests during pregnancy to facilitate the health of the pregnant woman and the growth and development of the fetus.