Antinuclear antibodies 1:100, granular and nucleolar generally refer to the titer of the antinuclear antibody as well as the type of antinuclear antibody. A titer of 1:100 is indicative of weak positivity. The granular and nucleolar types refer to the type of antinuclear antibody and mostly indicate the distribution of the antinuclear antibody in the nucleus of the cell. Antinuclear antibody 1:100 indicates the titer of antinuclear antibody, which is weakly positive. When the titer is greater than 1:80, it is positive for antinuclear antibody, and the higher its titer, the greater the correlation with autoimmune disease. Antinuclear antibody is a group of autoantibodies with different clinical significance, and its fluorescence nuclear type is classified into five types: homogeneous, granular, nuclear membrane, nucleolus and filament type. Granular type refers to the scattered bright fluorescent particles seen in immunofluorescence detection of antinuclear antibody, which is caused by anti-ENA antibody; high titer is commonly seen in mixed connective tissue disease, and also in autoimmune diseases such as systemic lupus erythematosus, scleroderma, and desiccation syndrome. Nucleolar type refers to fluorescence staining of the nucleolus only, and its associated antibodies are anti-nucleolar specific low molecular weight RNA, etc. High titers are specific for diagnosing scleroderma, but can also be seen in Raynaud’s phenomenon, and occasionally in systemic lupus erythematosus. When abnormal antinuclear antibodies are found, it is recommended to go to a regular hospital in time, complete the relevant examinations to clarify the cause of the disease, and follow the doctor’s instructions to standardize the treatment.