Requirements for sex hormone 6 assay
1. No sex hormone drugs have been used for at least 1 month prior to the serum reproductive hormone test to avoid affecting the test results (except for re-examination after estrogen and progesterone treatment or ovulation promotion therapy). In cases of scanty menstruation or amenorrhea, if the urine pregnancy test is negative, if there are no follicles >10mm in the ovaries bilaterally on vaginal ultrasound, and if the endometrial (EM) thickness is <5mm, this can also be considered as the baseline status.
2. Examination according to clinical needs
(1) Basal sex hormones: Measurement of sex hormones on 2-5 days of menstrual cycle is called basal sex hormone measurement. Basal LH, FSH and E2 should be measured on days 2 to 5 of the menstrual cycle, with day 3 being the best; if the cycle is shorter than 28 days, the examination time should not exceed day 3, and if the cycle is >30 days, the examination time should not exceed day 5 at the latest. Prolactin (PRL) and testosterone (T) can be measured at any time of the menstrual cycle.
(2) Late follicular phase (D12-16): E2, LH and P are measured when the follicles are near maturity to predict ovulation and the timing and dosage of HCG injection; P value is measured to estimate endometrial tolerance.
(3) PRL measurement: can be measured at any time of the menstrual cycle, blood should be drawn at 9 to 11 a.m., on an empty stomach, in a quiet state. a single test can determine significantly elevated PRL, while a second test should be performed for mild elevations. hyperprolactinemia (HPRL) should not be easily diagnosed and abused with bromocriptine treatment.
(4) Androgens: The commonly used tests are serum testosterone, androstenedione, and dehydroepiandrosterone sulfate. The significance of testosterone alone is small, and the biochemical indicators for evaluating hyperandrogenemia mainly rely on free testosterone.
(5) P: Luteal phase measurement (D21-26 days) is chosen to understand ovulation or not and luteal function.
This article is authorized by Dr. Zhang Xinshou.