The significance of cytokine 12-item test includes detecting inflammation and immune regulation, assisting in early diagnosis and prognosis assessment of tumor patients. Cytokines are small molecule peptides, which can be secreted by various cells, including interferon, interleukin, colony-stimulating factor, tumor necrosis factor and so on. 1.Detection of inflammation and immune regulation: a variety of cytokines are involved in the occurrence of inflammation and immune regulation, therefore, changes in the level of cytokines can be used to detect inflammation and immune regulation, such as the level of interleukin-6 and interleukin-10 can assist in the diagnosis of infectious pathogens as Gram-positive or Gram-negative, and the level of tumor necrosis factor can assist in the diagnosis of viral infections or tuberculosis infections. When graft-versus-host disease occurs after transplantation, the level of interleukin will also increase significantly, suggesting that the immune regulation function is abnormal. 2. Assisting in early diagnosis and prognosis of tumor patients: tumor development is often accompanied by increased levels of inflammatory factors, while the immune function of tumor patients is suppressed, and the levels of cytokines related to immune regulation will decrease, so the changes in cytokine levels can be detected to monitor the changes in patients’ conditions. The significance of cytokine 12 test is mainly related to inflammation, immune system and tumorigenesis, and its specific clinical significance should be based on the results of different diseases of patients. Therefore, patients should consult their doctors for further examination or treatment after receiving the cytokine 12 test results.