The cause of tympanic membrane invagination is the obstruction or narrowing of the Eustachian tube, which causes the tympanic membrane to deviate from its normal position, thus triggering the depression of the tympanic membrane into the tympanic cavity, and it is recommended that the patient consult a doctor in time for medical treatment. The Eustachian tube is a tube that connects the tympanic chamber with the nasopharynx. Air enters the tympanic chamber from the pharyngeal orifice through the Eustachian tube to keep the air pressure in the tympanic chamber the same as that of the outside world, so that the tympanic membrane can maintain its normal position and function. Tympanic membrane invagination can be caused by inflammation of the Eustachian tube, metamorphosis, air pressure injury and other reasons, such as acute eustachian tube inflammation, adhesive otitis media, chronic otitis media, air pressure injury otitis media, etc. Due to the swelling of the mucous membrane of the Eustachian tube, the lumen of the Eustachian tube becomes narrowed or blocked, resulting in the Eustachian tube is not clear, when the gas inside the tympanic chamber is absorbed the pressure can be lowered, which will lead to the tympanic membrane invagination. Patients with tympanic membrane invagination should consult a doctor in time and complete relevant examinations under the doctor’s guidance, such as otoscopy, audiometry, acoustic impedance, etc., so as to find out the cause of the disease and carry out appropriate treatment.