Botulinum toxin (Botulinumtoxin, BTX) is a neurotoxin produced by the bacterium Clostridium botulinum that inhibits neurotransmitter release and has a strong muscle relaxing effect. It is not only available as a cosmetic injection as it is often known, but in our hospital BTX injection therapy has been used as a major adjunctive therapy to create favorable conditions for muscle relaxation in adult patients with hemiplegic or paraplegic myospasm, as well as rehabilitation training for children with spastic cerebral palsy, thus facilitating functional training to correct abnormal posture, improve motor function, and enhance quality of life. At present, BTX-A injection positioning techniques mostly use freehand positioning, electrical stimulation, EMG guidance and ultrasound positioning, among which our hospital carries out ultrasound-guided positioning for puncture injection. Ultrasound-guided positioning allows visual observation of the needle tip position and avoidance of blood vessels and nerves, which is especially suitable for fine and complex upper limb muscles as well as deep muscles of the lower limbs. The functional department of 81 Rehabilitation Center adopts ultrasound localization technology to cooperate with clinical treatment of spasticity of upper or lower limbs in adults with hemiplegia or paraplegia, spastic muscle groups of upper and lower limbs in children with cerebral palsy and injection of salivary glands in children with salivation, with remarkable efficacy and 100% efficiency.