Post-exposure prophylaxis for hepatitis B. Accidental post-exposure prophylaxis When there is accidental exposure of broken skin or mucous membranes to the blood and body fluids of HBV-infected individuals, the following methods can be followed for treatment:20 1. Serologic testing: HBV DNA, HBsAg, anti-HBs, HBeAg, anti-HBc, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) should be tested immediately and retested within 3 months and Active and passive immunization: If you have received hepatitis B vaccine and are known to be positive for anti-HBs, you may not be given special treatment. If you have not received hepatitis B vaccine, or if you have received