How much do you know about the function of the gallbladder, and how harmful is it to the body after removal? The storage of bile is the most basic and well-studied function of the gallbladder. The gallbladder can be compared to a “reservoir” in which most of the bile produced by the body is stored for future use; studies from the first gallbladder removal in the late 19th century to the present have found that the loss of the gallbladder’s storage function after surgery does not seem to have much effect on the body. However, the emptying function of the gallbladder is another important function of the gallbladder. The role of gallbladder emptying function on the flow of bile and the effect of uninterrupted flow of bile into the intestine after cholecystectomy on the physiological changes in the gastrointestinal tract still need to be further investigated. It has been clinically found that bile reflux gastritis, abdominal pain and discomfort, and increased incidence of colon cancer after cholecystectomy may be related to the above loss of gallbladder function. Therefore, for the function of gallbladder, we should pay more attention to the emptying function of gallbladder and the effect of bile on the physiological changes of digestive tract. Because this may be one of the factors that potentially affect the physiological changes of the body after gallbladder removal and may be one of the risk factors for the development of tumors in the digestive tract.