The order of reporting of hepatitis B two-and-a-half in the general hospital laboratory test is hepatitis B surface antigen HBSAg, hepatitis B surface antibody HBSAb, hepatitis B e antigen HBeAg, hepatitis B e antibody HBeAb and hepatitis B core antibody HBcAb, let’s analyze the significance of these five tests in turn. HBSAg: positive indicates hepatitis B virus infection, active and inactive, inactive HBSAg carriers (HBSAg positive, HBeAg negative, HBeAb positive or negative), hepatitis B virus DNA below the lower limit of detection or <200IU/mL, more than 3 consecutive follow-ups within 1 year, each at least 3 months apart, ALT and AST in normal range . HBSAb: i.e. anti-HBs, a protective antibody, whose positivity indicates immunity to HBV, seen in hepatitis B recovery and hepatitis B vaccination recipients. HBeAg: represents hepatitis B virus replication, and higher quantification indicates more active replication. HBeAb: i.e. anti-HBe, in one case it indicates that viral replication is inhibited; in another case, the hepatitis B virus has undergone genetic mutation and is unable to produce e antigen, but viral activity has not decreased, which case needs to be judged according to the hepatitis B virus DNA test. HBcAb: that is, anti-HBc, the total antibody is mainly IgG type antibody, as long as the infection has been HBV, regardless of whether the virus is cleared, this antibody is mostly positive, and very few will also disappear. In summary, different combinations of the five hepatitis B items are commonly used clinically to determine the status and regression of infection. However, in addition to the hepatitis B virus infection, patients may also require DNA quantification, liver function, methemoglobin, abdominal ultrasound, and, liver fibrosis indicators to comprehensively assess the patient's condition.