A negative Hepatitis B test is a negative Hepatitis B 2.5 test result, indicating that the person being tested is not infected with the Hepatitis B virus. Generally, hepatitis B virus testing is available for high-risk groups and immunocompromised individuals. Hepatitis B test is performed by drawing blood for laboratory tests, and its test item is Hepatitis B 2.5, which is a serological marker for detecting hepatitis B virus in the patient’s blood, belonging to the three antigen-antibody system. The five indicators of hepatitis B include hepatitis B virus surface antigen and hepatitis B virus surface antibody, hepatitis B virus e antigen, hepatitis B virus e antibody, and hepatitis B virus core antibody. If all five indicators of hepatitis B are negative, it means that the examiner is not infected with hepatitis B virus, and there is no immunity to hepatitis B virus, and he/she belongs to the susceptible group of hepatitis B virus. It is recommended that these people should be actively vaccinated against Hepatitis B virus. Newborns, infants and children under the age of 15 who have not been immunized need to be actively vaccinated against hepatitis B according to their own conditions, which can effectively prevent hepatitis B virus infection.