BACKGROUND: Living liver transplantation techniques are becoming more sophisticated, but the incidence of biliary complications is still higher than that of cadaveric liver transplantation. Intraoperative cholangiography techniques have not reduced this complication and new preoperative evaluation and intraoperative surgical techniques are considered. STUDY OBJECTIVE: To innovate a new technique without cholangiography for living liver transplantation. METHODS AND DATA: Between September 2007 and May 2008, 21 living liver transplants between right hemihepatic adults were performed, all without intraoperative cholangiography. In all cases, preoperative iodothyronine (a new ion contrast agent) was administered intravenously, and high-resolution CT with the MeVis software analysis system (MeVis Technology, Bremen, Germany) was used to clearly visualize the structure of the biliary tree above the secondary bile ducts and to determine the correct resection line. All cases were followed up for more than six months. Results: Among the 21 right hemihepatic grafts, 14 contained one bile duct, 5 contained two bile ducts, 2 contained three bile ducts, 5 had multiple bile duct reconstructions, and 2 had bile-intestinal anastomoses. 4 of the 21 cases had biliary fistulas, and no biliary strictures were formed in the recent follow-up. 3 cases had immediate postoperative ERCP bioadhesive closure, and 1 case had a new bile-intestinal anastomosis. The incidence of biliary complications was 19%. CONCLUSION: With the right hemihepatic as the graft, the MeVis technique can avoid intraoperative cholangiography. Preoperative familiarity and mastery of intrahepatic bile duct anatomy is required to develop the correct resection line. The correct resection line and microsurgical reconstruction technique are the keys to reduce biliary complications.