In recent years, due to the gradual increase in the incidence of lung cancer, it has become one of the major diseases of malignant tumor death. The differentiation between lung cancer and benign lesions cannot be based on imaging as the main diagnostic basis, and pathology is still the gold standard for the diagnosis of lung cancer, and CT-guided percutaneous lung puncture biopsy can accurately show the location of the tumor and the anatomical relationship with the surrounding tissue structures, provide safe access to the needle site, angle and depth, and cause very little pain to the patient. It is also possible to reach the ideal pathological tissue. Good indications are the prerequisite for successful surgery to ensure safety: 1. Vascular lesions, coagulation disorders, severe chronic pulmonary emphysema, severe pulmonary fibrosis, pulmonary hypertension, puncture through pulmonary alveoli or cysts, and cachexia are all contraindications to lung puncture biopsy. 2, routine preoperative examination of coagulation routine, blood type, blood routine, electrocardiogram, preoperative sedative cough medicine for nervous or coughing patients, active communication with patients to eliminate their fear and active cooperation with the operator. Open intravenous access, prepare hemostatic drugs and routine resuscitation drugs before surgery. 3.Select a position that is comfortable for the patient, convenient for fixation and conducive to puncture, and shorten the operation time as much as possible. 4. Ask the patient to breathe steadily during the localization scan; the puncture process should be performed under steady breathing to avoid damage to the pleura and deviation of small nodule localization caused by respiratory movement. 5.Select the nearest point of the lesion from the near chest wall to enter the needle, avoiding the ribs, scapulae and important organs. 6.The puncture needle should be 18G. If the needle is too thick, the damage to the lung tissue is heavy, and it is easy to have complications of pneumothorax and coughing up blood; if the needle is too fine, the specimen is too small, which affects the pathological diagnosis. 7, lesions with necrosis, try to take the material at the edge of the lesion. 8, puncture specimens with 10% formaldehyde fixed sent to the pathological examination. The most common complications of percutaneous lung puncture biopsy are pneumothorax and pulmonary hemorrhage, pneumothorax amount within 30%, no obvious symptoms, more do not need to deal with, greater than 30% can be closed drainage of the chest, generally about 48 hours to pull out the drainage. Generally speaking, the smaller the lesion, the farther it is from the chest wall, the more difficult it is to puncture, and the thicker the puncture needle, the more likely it is to cause pneumothorax; pneumothorax almost never occurs when the needle is inserted from the place where the lesion is close to the chest wall. The operator is not experienced enough to try to take the vertical needle approach. The vast majority of pulmonary hemorrhage is intrapulmonary, only after puncture CT scan lung window shows distribution along the needle tract or a few thin patchy or cloudy shadows at the edge of the lesion, which can be observed. If more blood is coughed, posterior pituitary hormone can be given intramuscularly and the bleeding can be stopped in about 10 minutes. Complications such as hemoptysis, subcutaneous emphysema, needle tumor implantation, mediastinal emphysema, air embolism, etc. are rare with percutaneous lung aspiration biopsy, and most of the literature also suggests that no lung cancer dissemination or implantation metastasis will occur. In conclusion, CT-guided percutaneous lung aspiration biopsy is a minimally invasive, safe, and accurate examination method with simple operation and few complications, which is acceptable to most patients and is extremely helpful for qualitative diagnosis, treatment planning, and prognosis of occupied lung sites.