Testicular puncture biopsy is the observation of pathological tissues by puncture and is used to diagnose testicular diseases. With the advent of minimally invasive puncture techniques, testicular puncture biopsy has become less invasive and quicker to recover, and has been widely used in the diagnosis and treatment of various patient conditions. Testicular puncture is divided into two main parts: male infertility and consideration of testicular tumors. 1, Varicocele preparatory surgery, testicular biopsy can determine the prognosis 2, semen examination for oligospermia, follicle stimulating hormone value in the normal range, testicular biopsy can be used to determine the spermatogenic function. 3, semen examination for azoospermia, testicular puncture biopsy can identify whether it is obstructive or testicular spermatogenic dysfunction. If the testicular volume is less than 10 ml, poor elasticity and significantly higher serum FSH level, testicular spermatogenesis failure can be diagnosed without testicular puncture biopsy. 4.Vasectomy shows obstruction of the vas deferens, before performing recanalization, while making testicular puncture biopsy to clarify testicular spermatogenic dysfunction. 5.It is used to evaluate the efficacy of hormone medication before and after treatment. 6.Testicular puncture biopsy combined with endocrine examination can determine whether the testicular hypogonadism is primary or secondary. 7.Cryptorchid fixation in late development of pubertal futures and testicular puncture biopsy can evaluate spermatogenic function to exclude malignant changes. 8.Testicular tumor is considered. Testicular puncture biopsy can help with early diagnosis.