With the enrichment of people’s medical and health care knowledge, the requirements for disease treatment are getting higher and higher, and the treatment of diseases requires minimal trauma to the body and impact on the quality of life. In recent years, the incidence of gallbladder stones has gradually increased, and the concept of small incision transendoscopic biliary stone extraction is proposed. After more than 10 years of exploration and research, we have achieved great success. Compared with cholecystectomy surgery, it preserves the function of organs, greatly reduces the complications of surgery and postoperative adverse reactions, and the quality of survival is significantly higher than that of cholecystectomy patients. According to statistics, the recurrence rate of gallbladder stones after cholecystectomy is only 4.39%. Removing the stones and preserving the gallbladder can not only treat gallstone disease, but also preserve the function of the gallbladder and avoid complications such as biliary tract injury. With the widespread use of laparoscopic technology, Dr. Jingqiang Zhou, chief physician of general surgery department of our hospital, has adopted two or three holes of trans-laparoscopic biliary stone extraction for the treatment of gallbladder stones by repeatedly exploring the combined application of laparoscopy and cholangioscopy, which does not require any incision and is done only through 2-3 0.5-1.0 cm perforations, greatly reducing the surgical It greatly reduces the trauma and pain of the patient, and receives very satisfactory results. After repeated exploration, on this basis, we successfully carried out “single-hole trans-laparoscopic ultrasonic lithotripsy for biliary stone retrieval”, which is performed only through a 1.0 cm puncture hole in the abdominal wall, and the stones in the gallbladder are removed by ultrasonic lithotripsy. Based on the minimally invasive two- and three-hole trans-laparoscopic biliary lithotomy, this method significantly reduces the surgical trauma, shortens the operation time, and reduces the patient’s pain to a greater extent, especially for the sediment-like stones in the gallbladder.