Amniocentesis is recommended for a third child at age 35 to rule out the risk of chromosomal abnormalities in the fetus. Pregnancy at the age of 35 is already an advanced maternal age, and due to the increased risk of chromosomal disorders in the fetus, it is recommended that pregnant women undergo prenatal diagnosis such as amniocentesis at the 16th to 22nd week of gestation to definitively rule out the possibility of chromosomal disorders in the fetus in combination. However, since amniocentesis is an invasive procedure, some older pregnant women may choose to undergo prenatal screening methods such as non-invasive prenatal testing technology. Non-invasive prenatal testing (NIPT) is a prenatal screening method that screens for common aneuploid chromosomal abnormalities by screening for fetal-derived free DNA (deoxyribonucleic acid) information in the plasma of pregnant women. However, NIPT screening has limitations and should not be used in special circumstances such as the possible presence of genetic disorders or structural malformations of the fetus.