Due to the influence of disease factors such as pediatric rabies, mania alternating with lethargy and cranky abnormality during attacks occur. Rabies is a zoonotic acute infectious disease of the central nervous system caused by rabies virus. What items are included in the examination of alternating mania and lethargy? 1, blood routine, urine routine and cerebrospinal fluid examination The total number of peripheral blood leukocytes (12-30) × 109/L varies, neutrophils generally account for more than 80%, urine routine examination can be found in mild proteinuria, occasionally transparent tubular, cerebrospinal fluid pressure can be slightly increased, the number of cells slightly increased, generally not more than 200 × 106/L, mainly lymphocytes, increased protein, up to 2.0g/L Protein is increased, up to 2.0g/L or more, sugar and chloride are normal. 2.Virus isolation Saliva and cerebrospinal fluid are commonly used to isolate virus, and the isolation rate of saliva is higher. 3.Antigenic examination Immunofluorescence examination is performed by skin or brain biopsy. 4.Nucleic acid determination The PCR method is used to determine RNA, and the positive rate is higher for saliva, cerebrospinal fluid or skin tissue specimens with hair follicles on the back of the neck. 5.Animal inoculation Specimens are inoculated in mice and then brain tissue is taken for immunofluorescence test to detect pathogens and pathological sections are made to examine Negri vesicles. 6.Antibody test is used to detect IgM in the early stage. 50% of the sera are positive at 8 days after the disease, and all of them are positive at 15 days. Serum neutralizing antibody was measured on 6 days after the disease, and the potency of neutralizing antibody can reach thousands after cellular vaccination, not more than 1:1000 after vaccination, while patients can reach more than 1:10,000.