Atypical squamous epithelial cells (ASC) and atypical glandular cells (AGC) are the most difficult of cervical cytopathology findings to determine the response. In this study, high-risk factors were recorded and colposcopic multi-point biopsies were performed in patients with both TCT results of ASC and AGC, and histopathological findings were used as diagnostic criteria for retrospective analysis to investigate clinical management options for TCT results of atypical epithelial cells (ASC+AGC). Cytological diagnosis Cervical cytology was analyzed using the Thin prep liquid-based cytology technique in the descriptive diagnostic manner of the TBS system of 2001. Colposcopy All patients had 2 dedicated physicians performing colposcopy, using the 2002 version of the New International Colposcopic Terminology to describe what was seen microscopically. Acquisition of histological specimens and pathological diagnosis Colposcopy was satisfactory for abnormalities in the cervical transformation zone, with multi-point biopsies taken from the abnormal area; for normal cervical transformation zone, biopsies were routinely taken at points 3, 6, 9 and 12. According to the degree of lesions, they were classified as chronic inflammation, cervical intraepithelial neoplasia mild (CIN1), cervical intraepithelial neoplasia moderate (CIN2), cervical intraepithelial neoplasia severe (CIN3), and cervical cancer (Ca). Documentation of high-risk factors A follow-up card was created for all patients to record high-risk factors, TCT, colposcopy, and cervical biopsy results. High-risk factors included: leukorrhea, cervical erosion, number of pregnancies, number of deliveries, age at first intercourse, and number of sexual partners. Data organization and statistical methods The cervical histological diagnosis was used as the gold standard for statistical analysis, and the results were entered in EXCEL for weight and error checking. The count data were analyzed by χ2 test, and the obtained high-risk factors were analyzed by SPSS10.0 statistical package, and the risk OR (odds ratio, ratio of numbers) was calculated.