It is necessary to check for infectious diseases such as hepatitis B, syphilis, and AIDS before performing a bone puncture. Clinically invasive maneuvers must be checked for infectious diseases such as hepatitis B, hepatitis C, syphilis, and AIDS before the procedure. Bone marrow aspiration belongs to one of the invasive maneuvers in the clinic, the main purpose is to take bone marrow fluid to achieve the purpose of diagnosis, such as unexplained red blood cells, white blood cells, platelets, the number of increased, decreased or even abnormal morphology of the disease caused by the clinic can be carried out after the bone marrow aspiration bone marrow smear, bone marrow culture and other tests to diagnose the disease. Bone marrow aspiration is mainly divided into iliac crest aspiration, vertebral spinous process aspiration and sternotomy according to the different parts of the puncture site. After the end of the puncture, it should be locally sterilized, then pressed to stop the bleeding and rest in bed for one day, and it should be done with caution for women who have a tendency to bleed, coagulation disorders, or late pregnancy.