1. abstain from sex (including no seminal emission or masturbation) for 3-7 days before collecting the specimen (2-3 days for those under 30 years old, 3-5 days for those 30-40 years old, 5-7 days for those over 40 years old) 2. discharge all the semen into a clean and dry container 3. adopt a relatively quiet and safe environment for sperm collection as far as possible 4. prohibit the use of condoms to collect the specimen and prohibit the interruption of sexual intercourse method 5. pay attention to heat preservation when it is cold Zhejiang 6. Use sterile containers for microbiological examination. 7. Bring the specimen to the laboratory within half an hour in a close environment. 8.