1.How can assisted reproductive technology help azoospermia patients to have children? Sperm is obtained through epididymal puncture/testicular puncture/microscopic sperm retrieval and then frozen sperm is used for IVF; if sperm cannot be obtained, donor insemination or donor IVF is recommended. 2. How much money should a couple who needs second-generation IVF prepare? What is the success rate? The cost of second-generation IVF is around 30,000-50,000; the success rate should be evaluated with the specific situation of the woman, usually around 50%-60% for women with normal ovarian function. 3.Before doing IVF, what materials should be prepared and which materials patients tend to miss to bring? Firstly, both parties should have their ID cards, marriage certificates and family planning service certificates; secondly, follow the medical advice of female and male doctors for IVF pre-operative examinations. 4.Should the female partner register with the gynecology department or the reproductive medicine department for the IVF procedure? The female partner should consult the fertility center/reproductive medicine department. 5.How long should the male partner have the sperm retrieval procedure after the female partner enters the process? It is usually recommended for the male partner to have the sperm retrieval procedure before the female partner enters the process. 6.How many times can the sperm and eggs be removed for IVF and how long can they be frozen respectively? The more sperm and eggs, the more times they can be used; as there are national regulations that do not allow long-term preservation of sperm and eggs in fertility centers that do not have human sperm banks, they are generally not preserved for a long time. 7.Does freezing damage the sperm and eggs? Nowadays, the technology of freezing sperm and eggs is very mature and there is usually no need to worry about it, usually the thawed sperm and eggs can be used for IVF normally. 8. If the success rate of sperm retrieval for the male partner is low, do you think sperm retrieval or egg retrieval should be done first? It is recommended to do sperm retrieval for the male partner first and then egg retrieval for the female partner.