The diagnosis of azoospermia must be confirmed by three different times of semen routine. One test is prone to misdiagnosis. Patients with azoospermia can be divided into the following categories: 1. Small testes and high blood follicle stimulating hormone (FHS) or luteinizing hormone (LH) on examination. This generally suggests that the testes are not spermatogenic, and even if the testes are biopsied there is usually no sperm. If the testicular volume is less than 5ML, FSH value is twice the upper limit of normal and testicular biopsy is not recommended. 2. The testes are normal in size and the blood follicle stimulating hormone (FHS) or luteinizing hormone (LH) is normal. Biopsy can be considered. 3.Low follicle stimulating hormone (FHS) or luteinizing hormone (LH) often indicates that the lesion is in the brain. Chorionic gonadotropin and urotropic gonadotropin can be used to achieve fertility in some patients. Treatment principles: 1, no sperm in semen, sperm found in testicular biopsy, can be solved by (ICSI) in vitro fertilization. 2. If there is no sperm in the semen and no sperm is found in the testicular biopsy, the sperm from the sperm bank will be used to achieve fertility. 3, drug treatment is only effective for some patients, most patients are ineffective, and the course of treatment is long. However, in clinical practice there are indeed some patients who have spermatozoa appear through Chinese and Western medicine treatment. In addition, testicular biopsy must be multi-point puncture biopsy. This is very important because the spermatogenic ability varies from one testicular area to another. Clinical observations suggest that azoospermia patients with varicocele have little clinical significance for high varicocele ligation.