The causes of lung cancer gene mutation mainly include: physical factors, chemical factors and biological factors. Physical factors, such as ultraviolet rays and ionizing radiation, may cause damage to genetic material, resulting in DNA damage and breakage, causing gene mutation. Chemical factors such as nitrites and hydroxylamine compounds can replace normal bases with incorrect base pairs, which can cause errors in the replication process and lead to gene mutations. Alkaloids may adulterate the DNA molecule, causing base complementary pairing errors; aromatic compounds may cause loss of bases or mismatches, potentially causing gene mutations. Biological factors mainly include herpes virus, influenza virus and various bacteria, which disrupt the normal replication of DNA while replicating the viral genetic material. Genetic mutations can be tested at a regular hospital.