Neomycin does not exist in the best combination, need to be rationalized according to the condition of the drug. Neomycin belongs to aminoglycoside antibiotics, in the clinic, neomycin is mainly used for colon surgery preparation, hepatic coma can also be used as one of the auxiliary therapeutic drugs. The optimal dosing of neomycin is generally understood to be a combination of drugs, but there is no optimal pattern of dosing, and there is also a large individual variability in the effects of the drug. Neomycin is effective against Gram-positive and Gram-negative bacteria, but has no significant effect on Pseudomonas aeruginosa. Improper use of neomycin may cause patients to experience adverse reactions such as tinnitus, dizziness, decreased urination, thirst, nausea and vomiting. Consultation with a doctor is recommended regarding the specific use of neomycin. In daily life, patients taking Neomycin need to strictly follow the doctor’s instructions for medication, so as not to cause other adverse effects.