The six female hormone tests include folliculogenic hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P), testosterone (T), and prolactin (PRL). 1. Distinguish two examination time points Hormone examination on the 2nd and 3rd day of menstruation (menstrual period): focus on ovarian reserve and basal endocrine level Hormone examination on the 12th and 13th day of menstruation (ovulation period): focus on follicular growth and maturation and ovulation 2. High FSH is seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. FSH higher than 40mIU/ml is not effective against ovulation stimulating drugs like clomiphene. (2) Basal E2 below 50pg/ml is normal: because E2 and FSH are negative feedback, even if the basal FSH is below 10, but E2 above 50pg/ml is equally likely to have poor ovarian reserve. (3) LH below 5 mIU/ml indicates gonadotropin insufficiency: high FSH combined with high LH is a clear sign of ovarian failure, and LH/FSH ≥3 is one of the bases for the diagnosis of polycystic ovary syndrome. (4) Prolactin above 17.6ng/ml is considered hyperprolactinemia: excess prolactin can inhibit the secretion of FSH and LH, inhibit ovarian function and suppress ovulation. (5) High testosterone, i.e. blood T value: hypertestosteronism can also cause infertility. In case of polycystic ovary syndrome, blood T value is also increased, hirsutism with acne, seborrhea and hair loss. Tests on the 12th and 13th day of menstruation (1) LH peak to determine ovulation: to see if there is a pre-ovulatory LH peak and to determine if you are close to/ or have ovulated, ovulation test paper is LH test paper. (2) E2 level to determine follicle quality and maturation time: normally a mature follicle has more than 150 estrogen as support, so as to judge the time of egg retrieval and injection of HCG follicle ripening injection. When the follicle size reaches 18 or more, but the estrogen is less than 150, it is considered as low estrogen and there is a possibility of empty follicles or poor egg quality. (3) Low progesterone may appear as ovulatory bleeding: low blood progesterone value in late ovulation is seen in luteal insufficiency, ovulatory type dysfunctional uterine bleeding, etc. Warm reminder: the above is only general experience, patients should follow the doctor’s instruction while in the hospital.