In recent years, the plastic surgery clinic face and neck wrinkles seekers (including head lines, crow’s feet, frown lines, neck wrinkles, nasolabial folds, etc.); bite muscle hypertrophy require thin face, calf muscle hypertrophy require thin calf seekers, palms and other special parts of hyperhidrosis patients and the first treatment, other treatment after the relapse of the treatment of axillary odor patients gradually increased. With the development of the national economy, the demand for minimally invasive cosmetic surgery has increased, and the number of patients requiring non-surgical treatment has also increased. According to literature statistics, in recent years, botulinum toxin injection treatment ranks first in plastic and cosmetic surgery non-surgical treatment, botulinum toxin application principle: botulinum toxin is a Gram-positive anaerobic bacterium botulinum in the process of reproduction of an exotoxin, can be divided into A, B, C1, C2, D, E, F, G 8 types. type A botulinum toxin is not heat-resistant, and more sensitive to changes in pH. Therefore, botulinum toxin is packed in an empty vial under vacuum and stored at -5°C. Botulinum toxin type A is a biological agent with unstable properties and should be used as soon as possible if dissolved in physiological saline, and can be stored in the refrigerator for 4 hours after dilution at 2 to 8°C. Botulinum toxin type A weakens skeletal muscle by cleaving the presynaptic membrane of the synapse-associated protein SNAP25, which blocks the release of acetylcholine from motor neurons and repolarizes the posterior membrane, blocking muscle contraction and causing flaccid paralysis of muscles. However, the blocking effect of botulinum toxin does not affect the production of acetylcholine, and after several months, the resulting nerve junctions and axons replace the non-functional neuromuscular junctions, and nerve function is restored again. Botox acts on the frontal muscles, frowning muscles, orbicularis oculi, and cervical muscles to relax the muscles, reduce wrinkles, and treat facial and neck wrinkles (including headlines, crow’s feet, frown lines, neck wrinkles, nasolabial folds, etc.); acts on the bite muscle, and continuous injection treatment can make the bite muscle atrophy, so that people with bite muscle hypertrophy can achieve the purpose of slimming the face; acts on the gastrocnemius and flounder muscles to make people with calf muscle hypertrophy achieve the purpose of slimming the calves; acts on the sweat muscle, and can make people with calf muscle hypertrophy achieve the purpose of slimming the legs. The purpose of leg; acting on sweat glands and other target organs, can make the palm of the hand and other special parts, the axillary area sweat gland atrophy, secretion of sweat to reduce the treatment of hyperhidrosis patients, as well as the first treatment, other treatment after the relapse and then treatment of patients with axillary odor . I. Drugs: Botulinum toxin type A for injection (Hengli), produced by Lanzhou Institute of Biological Products, approval number: S10970037, specification: 100IU/bottle. Botulinum toxin type A for injection, produced by GlaxoSmithKline, with the approval number of S20070023 and the specification of 100IU/bottle. 2, application experience and precautions: 1, uniform and symmetrical distribution of the drug, mark the location of the injection points and the number of injection points before injection, according to the number of injection points, the average distribution of each injection amount; 2, the depth of injection should be appropriate, generally inject the subcutaneous muscles, sweat glands are located in the level, the location of the expression muscle is shallow, the bite muscle, gastrocnemius muscle is generally hypertrophic, sweat glands are located deeper, located in the subepidermal 1.7 ~ 3.7 3, the needle into the injection depth and level of adjustment, back to draw no blood after slow and even injection, in order to avoid the drug into the blood after the increase in the probability of adverse reactions, while the injection of the drug into the veins increased the production of antibodies, thus affecting the effect of the second injection; 4, after the injection to avoid local squeeze To avoid leakage of the drug, care should be taken not to make the eye of the needle from the skin to the subcutaneous located in a straight line, the method is to move the needle around slightly after the needle is inserted obliquely and then punctured to the deeper layer, the cotton swab is lightly pressed to stop the bleeding after the needle is withdrawn and covered with sterile dressing; 5, the patient should be observed for 20-30 minutes after the treatment, no adverse reaction before leaving, avoid 6.Epinephrine and dexamethasone should be routinely available for first aid in case of occasional allergic reactions; 7.The interval between two injections should be at least 3 months, and it is generally recommended that the patient should have an interval of less than 6 months, so that the chance of antibody production will be reduced and antibody neutralization and reduced efficacy can be avoided when injecting again. In conclusion, botulinum toxin local injection treatment has long-lasting and precise efficacy, low recurrence rate, easy to operate, light tissue damage, little pain, does not affect tissue morphology and function, does not affect daily life and work, can be repeatedly injected, suitable for patients who do not want to accept surgical treatment or patients who receive laser, drug or surgical treatment relapse, safe and effective, worthy of clinical It is safe and effective and worthy of clinical application.