Nucleic acid testing is not painful. Nucleic acid testing is currently performed by means of a pharyngeal swab, which means that the patient opens his or her mouth, exposes the throat, and swabs the tonsils and the back wall of the pharynx with a designated swab for specimen collection, and when the secretion is collected, a special test tube and specimen collection bag are placed, stored at low temperature, and then sent to a testing facility in a timely manner. The nucleic acid test is not painful, but there is a possibility of dryness and vomiting. Therefore, when performing nucleic acid testing, health care workers should wear secondary protection, such as face screens, N95 masks, eye protection, and disposable isolation gloves. As the person whose specimen is collected, it is best to hold back from choking and vomiting to avoid the risk of droplet transmission.