There is no difference between a morning and afternoon testosterone test for the sex hormone six, but afternoon testing is not recommended. The sex hormone six test for women is usually performed within 3 to 5 days of the menstrual period, as this is the time that accurately reflects the basal function of the ovaries. There is no time difference in the factors affecting testosterone production, so the morning or afternoon of the day has no effect on testosterone test results. However, other items in the sex hormone test, such as prolactin, fluctuate significantly at different times of the day, so it is often recommended that the test be done on an empty stomach from 9 to 11 a.m. in order to have an accurate test result as well as a convenient visit to the doctor. It is recommended that the test be performed by a doctor and that you follow the doctor’s instructions on when to take the test.