Lung cancer puncture is percutaneous, trans-CT guided puncture of intrapulmonary lesions, which means that the location, puncture angle and direction of the lesion are observed under CT guidance, and a needle is used to pierce through the skin into the lung tissue and make a puncture in the direction of the tumor. In addition, transbronchoscopic puncture is required for tracheoscopy if the lung lesion grows near the hilum, i.e., central type lung cancer. Tracheoscopy is a thin tube inserted from the nose, which can observe the specific location and shape of lung cancer under imaging guidance, especially under direct vision, and clip part of the tissues through the specific shape to do pathological laboratory tests, and the purpose of puncture is to do pathological laboratory tests. In addition, some patients may have lymph node metastasis, such as supraclavicular lymph node metastasis, which is relatively superficial, and the puncture requirements are relatively simple, so the puncture can be done under ultrasound guidance avoiding blood vessels and important organs. Whether by CT, percutaneous lung puncture, transbronchoscopic lung puncture, lung puncture in the lymph nodes, or other distant sites, mainly a small portion of tissue is removed, about 2-3g in weight and 1cm. The tests can provide greater help, including the type of pathology, genetic type, and medication. Therefore, the vast majority of lung cancer patients need to be diagnosed clearly by puncture and choose better treatment.