The entire process of donating peripheral blood for HSC can be divided into the following ten steps:
1. Application for banking
When a person who meets the entry requirements wants to donate blood stem cells, he or she must first go to a blood donation site or blood stem cell donation center and its branches with the appropriate conditions, fill out the Consent Form for Voluntary Blood Stem Cell Donation and the Blood Stem Cell Donation Registration Form and other relevant information, and leave about 8 ml of blood sample for testing such as human leukocyte antigen (HLA) typing. In addition to meeting health standards, the application to fill out the registration form should confirm that the donor has a more detailed understanding of donating blood stem cells, has given it a lot of thought, and is able to fulfill the donation commitment after the match is made.
2. Information and test data entry
The Blood Stem Cell Donor Bank Management Center and its branches will send blood samples to designated laboratories for testing as soon as possible, and then enter information such as qualified volunteer donor information and HLA typing test data into the China Volunteer Blood Stem Cell Donor Bank, waiting for patients who need a blood stem cell transplant to retrieve a match. From then on, the applicant is truly a volunteer blood stem cell donor in the Chinese bone marrow bank. Thereafter, volunteer donors should keep in touch with the database management and update their contact information to ensure smooth communication.
3. HLA prime-match remobilization
When the blood stem cell donor database retrieves an HLA match between a volunteer donor and a patient in need of a bone marrow transplant, institutional staff will quickly contact the donor and, if it is confirmed that the donor is physically and mentally fit to continue to fulfill the donation commitment, remobilize the donor and explain the donation and collection methods and possible problems and solutions to be encountered during the collection process, hoping that the donor will We hope that they will actively cooperate.
4. Retain blood samples again for high-resolution matching compound testing
Professionals will need to take another blood sample from the volunteer donor and send it to a designated laboratory for HLA high-resolution compatibility testing. When the test determines that the HLA of the volunteer donor is compatible with the HLA of the patient requiring HSCT and meets the requirements for transplantation, the donor signs a consent form and agreement to donate, and a pre-collection physical examination is arranged by the repository management center and its affiliates at the designated hospital.
5. Pre-collection physical examination
The volunteer hematopoietic stem cell donor is evaluated by a specialist on the results of the physical examination and the physical and mental condition of the volunteer donor to confirm that the volunteer donor is eligible for hematopoietic stem cell donation, and the Chinese Bone Marrow Bank Management Center informs the above overall situation to the hospital that applied for a match for the volunteer donor and the patient, and then the transplant hospital, the database and the collection hospital work together to develop the collection and transplantation plan.
6. Injection of hematopoietic stem cell mobilizer
After the collection and transplantation plan is developed, the transplantation hospital, the repository, and the collection hospital proceed with simultaneous collection and transplantation preparation based on the plan, respectively. The proposed peripheral blood HSC donor needs to receive subcutaneous injection of HSC mobilizer (also called inducer, granulocyte colony-stimulating factor, G-CSF) at the collection hospital or collection center 4 to 5 days before collection to induce a large number of HSC from the red bone marrow into the peripheral circulating blood and to be monitored.
7. Collection of peripheral blood hematopoietic stem cells
Hematopoietic stem cell collection can be initiated when the density of hematopoietic stem cells in peripheral blood, has reached an ideal state, usually on the fifth day of hematopoietic stem cell mobilizer injection. The process of collecting HSCs is the same as that of blood donation, where a machine separates the blood flowing in the blood vessels and extracts the peripheral blood HSCs from it. The whole process takes about 3 to 5 hours, and the amount collected depends on the weight of the patient and donor and the amount of HSCs needed for transplantation.
If the patient needs a small number of HSCs and the donor is heavy, one HSC collection is sufficient; if the number of HSCs collected at one time does not meet the need, another collection will be made the next day, with approximately 200 mL of HSC suspension collected each time. Each therapeutic amount of HSC suspension usually contains 5 to 10 grams of HSCs.
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8. Post-collection observation
After the needle is removed at the end of the collection, continue to observe and rest as requested by the physician, and after 2 hours without abnormal symptoms, you can perform soothing activities around the blood collection room.
9. Follow-up
Retrospective physical examinations and follow-up visits will be performed according to the physician’s requirements and protocols.
10. Recognition, fellowship and volunteer service
When holding bone marrow and hematopoietic stem cell donations and other public service and charity events, honor should be honored and finances should be downplayed.
In 2008, the State Ministry of Health, the General Association of the Red Cross Society of China and the Ministry of Health of the General Logistics Department of the Chinese People’s Liberation Army honored those who had donated blood stem cells (including marrow donation) in previous years within mainland China, and each blood stem cell donor was awarded the National Award for Dedication to Blood Stem Cell Donation without Compensation. The company’s main goal is to provide a comprehensive range of products and services to the public.
Usually, the national authorities will hold a national blood donation recognition ceremony every two years.
The company’s main goal is to provide a platform for the development of a new generation of blood stem cell donors, and to promote the spirit and behavior of love and dedication.