As we mentioned last time, in most cases, the sperm and the egg cannot meet, so we can make them meet and unite them in vitro. However, there are still some patients whose sperm count or vitality problems can’t impress the egg at all, in which case we can only force them to combine, which can be regarded as “forced marriage”, that is, we are talking about the second-generation IVF – Intracytoplasmic Microprocessor Injection of Single Sperm (ICSI). ICSI). So, what is the whole process of “forced marriage”? The procedure of egg retrieval is exactly the same as that of the first generation, after the egg is taken out, the periphery of the egg is wrapped by a lot of granulosa cells, just like a thick armor, if we want to force the sperm to combine with the egg, we have to think of ways to get rid of that layer of armor, about 3 hours after the egg is taken out, we will use the enzyme hyaluronidase to take off the granulosa cells around the egg, and the egg which is deprived of the protection will feel slightly uneasy and panicky, and we have to let the egg take a little bit of time to be inseminated before fertilization. Before fertilization, we allow the egg to rest and calm down a little, this time is about 1-2 hours. Once the bride is ready, the groom will come on stage and inject the sperm into the egg with a hollow glass needle, much thinner than a hair, but as you know, sperm is like a tadpole, will he give in gently? Of course not, because it is not like free love two people love each other, then how to do it? We have to be a little bit in his little tail on the hands and feet, so that he can not escape the wedding scene, when the sperm was injected into the egg, generally cultivated to the third day after the egg transplantation back to the patient’s uterus. Whether voluntary or forced, the union of sperm and egg heralds the arrival of a new life!