Negative H. pylori suggests that there is no H. pylori infection. H. pylori can invade the pylorus of the stomach through fecal-oral transmission, thus destroying the protective barrier of the gastric mucosa and leading to the risk of gastritis and gastric ulcers. Common tests for H. pylori include the 13C or 14C urea breath test and the rapid urease test. When the test result is positive, it suggests the presence of H. pylori infection, and when it is negative, it suggests the absence of infection. It is important to note that false-negative results can occur with major bleeding in the stomach or with short-term use of medications such as antibiotics, bismuth, and acid-suppressing drugs. Therefore, it is important to stop taking antibiotics and bismuth 4 weeks before the H. pylori test, and stop taking acid suppressants 2 weeks before the test to avoid false negative results. If H. pylori is positive, it is recommended to seek prompt medical attention and regular treatment under the guidance of a doctor.