Hepatitis B vaccination does not cause an increase in the level of alpha-fetoprotein inside the body. Hepatitis B vaccine is a vaccine against viral hepatitis B. The vaccination stimulates the body to produce specific antibodies that effectively defend against the invasion of hepatitis B virus. The alpha-fetoprotein is a substance synthesized by newborn liver cells and disappears quickly after birth, so the alpha-fetoprotein contained in the body of a healthy adult is very small. Hepatitis B vaccination does not cause elevated alpha-fetoprotein. Elevated alpha-fetoprotein is primarily associated with pregnancy, active liver disease, hepatocellular carcinoma, embryonal carcinoma of the gonads, and other conditions. Individuals with abnormally elevated levels of alpha-fetoprotein are advised to seek medical attention to identify the cause.