Weakly positive cervical screening 16 requires regular follow-up if it is not accompanied by symptoms and histologic abnormalities of the cervix; if it is accompanied by cervical lesions and cervical cancer, surgical treatment is required. Weakly positive cervical screening 16 refers to cervical exfoliated cell screening, the results of which suggest human papillomavirus 16 infection. HPV16 is a high-risk type of HPV, which is likely to lead to cervical cancer, and needs to be combined with colposcopy, liquid-based thin-layer cytology, and, if necessary, pathology to clarify the presence of cancer. If the patient is only weakly positive for HPV16, without cervical histologic abnormalities or other symptoms, no special treatment is needed, and the immune system can usually clear the virus after 2 years of infection with regular follow-up examinations. If it is accompanied by vaginal contact bleeding, abdominal pain, vaginal bleeding and other symptoms, or clear cervical intraepithelial neoplasia or cervical cancer, then it is necessary to carry out cervical LEEP conization surgery or cervical cancer staging surgery and postoperative treatment with radiotherapy. Therefore, patients are advised to pay attention to personal hygiene, maintain good living habits, and follow the doctor’s instructions to improve further examination and treatment.