Nucleic acid testing usually involves the extraction of mucus and secretions from the nasopharynx or pharynx as specimens for testing. During the test, the mucous membrane of the nasopharynx or pharynx is irritated and dry heaving occurs, which is a reflexive symptom caused by local irritation during the collection and is generally unavoidable. Therefore, there is no technique for not dry heaving during nucleic acid testing, and only measures can be taken to relieve dry heaving symptoms. Do not eat two hours before the nucleic acid test, drink a small amount of water to moisten the mucous membrane of the throat area, and avoid taking medications. Understand the process of nucleic acid testing and the precautions to take, relax and avoid tension. When sampling, cooperate with the doctor to take samples, open the pharyngeal cavity correctly, tilt the subject’s head back when collecting oropharyngeal swabs, open the mouth and make an “ah” sound to help expose the throat, and during the collection process, nasal itching and sneezing may occur. When dry heaving occurs during the nucleic acid test, the degree of reaction varies from person to person, and can be slowly relieved with proper rest. In case of severe dry heaving, or if it causes vomiting and other physical discomfort, blood sampling can be used for testing. Of course, dry heaving is also related to the sampling technique of the sampler, but the sampling personnel are professionally trained, gentle and skillful sampling movements, can be completed in a relatively short time, the subject can eliminate concerns and actively cooperate.