The standard value for quantification of hepatitis B DNA should be <10³ copies/ml, and ≥10³ copies/ml is considered positive. Hepatitis B DNA (HBV-DNA) is a direct marker of viral infectivity and replication. Quantitative testing can determine the infectious size of the virus, the degree of replication, and so on. HBV-DNA <10³cps/ml is negative; HBV-DNA ≥10³cps/ml is positive, indicating the presence of HBV virus in the body, suggesting that it may be chronic hepatitis B patients, patients with occult hepatitis B, etc. HBV-DNA ≥10⁵cps/ml, e antigen negative and HBV-DNA ≥ 10 ⁵cps/ml indicates more active viral replication. The standard value of hepatitis B DNA quantification is also related to different testing methods, different laboratories, etc. It is recommended that patients combine with the reference value of local hospitals to make a comprehensive judgment, and if there is any abnormality, they should consult with a specialist and take active treatment.