Semen examination for male infertility patients is one of the most basic methods to check male reproductive function. Semen examination is easy to perform, the laboratory data is accurate and has a high clinical reference value, and it is also the first routine examination to be done for male infertility. The semen collection procedure in semen examination is very important and must be strictly observed according to the regulations in order for the test results to be accurate and valid. However, patients are often susceptible to interference from a variety of factors when doing semen tests. If the semen collection method is improper, not only will the accuracy of the test be affected, but there may even be significant deviations. Men who undergo semen testing should pay attention to the following points. First of all, abstinence for 3 – 7 days, in addition to the prohibition of conjugal intercourse and masturbation, should also avoid the occurrence of dream loss, which is to ensure that the amount of semen is sufficient during the examination and can objectively reflect the quality of semen. In addition, the length of abstinence is also important. If the time is too short, the semen volume and sperm density will be insufficient; if the time is too long, it will lead to a decrease in sperm motility and affect the accuracy of the examination. Secondly, semen examination should also be avoided during periods of poor health, such as after having a cold and fever or drinking a lot of alcohol. This may lead to low sperm or dead sperm, which may also affect the accuracy of the test. Again, when sampling sperm, it is best to do so on site in an outpatient clinic. Usually the semen examination room or outpatient bathroom is sampled with the masturbation method and sent for examination immediately after sampling, which can avoid semen leaving the body for too long or being contaminated midway. The entire semen should be collected when sampling, especially the first part of semen just after ejaculation. The semen should be contained in a clean container provided by the hospital, and condoms should not be used (condoms mostly contain lubricants or spermicides, which can affect the viability of sperm). If it is winter when the sample is taken, the container should be placed in the arms to keep warm and sent for testing. The appropriate temperature to maintain semen samples is between 20°C – 35°C. Finally, some patients need to be sampled at home due to psychological factors. In this case, the patient should send the sample for testing within the time specified by the doctor and should not leave it for too long.