Fertility is the business of both husband and wife, so when obstacles arise on the road to conception, both should be actively examined and treated. The following will highlight some of the male infertility checkup items, of course, not every patient needs to do these comprehensive examination, the general doctor will be based on the patient’s specific circumstances to choose the checkup items, so that not only can use the least amount of time and money to make the patient to a more accurate and more comprehensive information about the disease, which is conducive to the treatment. 1, medical history: to understand the time of infertility, primary or secondary infertility, occupation and type of work, past medical history, marriage and sex life, previous examination and treatment, family history. Physical examination: including general and reproductive organs examination, pay special attention to the development, nutrition and mental status, but the focus is on the examination of reproductive organs, including penis, urethra, prostate, testicles, spermatic cord and so on. Laboratory tests: semen analysis, prostate fluid examination. Semen examination is an important examination program to diagnose male infertility. It can reflect the quality of spermatozoa produced by testes, the smoothness of spermatic ducts and the secretion function of epididymal glands. 4, endocrine examination: through the examination can understand the function of testes. If necessary, thyroid hormone, adrenal sebaceous hormone or prolactin can be measured. Doppler ultrasonography: it helps to confirm the condition of varicocele, seminal vesicles, prostate and testicles. 6, immunological examination: through the sperm agglutination test or brake test to detect sperm agglutination antibody or brake antibody in serum or seminal plasma. 7. Testicular biopsy: It is used for azoospermia or oligospermia to directly check the spermatogenic function of testicular seminiferous tubules and the development of interstitial cells. It can know whether there are sperms in the testis or not. 8. Karyotype analysis: It is used for abnormalities of external genital organs, testicular dysplasia and azoospermia of unknown cause.