Steps in IUI Monitoring follicle development: IUI can be performed with either natural or ovulation-promoting cycles, and studies have found that ovulation-promoting cycles can increase pregnancy rates. A natural cycle involves monitoring follicle size and endometrium without medication. Ovulation cycles refer to the monitoring of follicles ≥ 18 mm with medications such as clomiphene, letrozole, and gonadotropins (HMG, FSH), and the administration of HCG to promote follicle maturation and rupture. IUI time selection: Ovulation time is determined by monitoring follicle development, and the IUI procedure is performed closest to female ovulation Semen processing: Fresh semen is collected by masturbation into a sperm collection cup, centrifuged by laboratory personnel, washed and then drawn into a syringe. Uterine Injection: The processed semen is passed through a catheter and injected into the uterine cavity. Factors affecting the success rate of IUI: Age of the couple: The increasing age of both spouses reduces the pregnancy rate. It is well known that a woman’s fertility decreases significantly with age, mainly due to a decrease in egg quality and a decrease in endometrial tolerance. Years of infertility: As the number of years of infertility increases, the chances of pregnancy decrease. Number of cycles of treatment: Most clinicians agree that couples who are infertile after 4-6 IUIs should undergo IVF treatment. Parameters of sperm: The viability of sperm and the percentage of normal form sperm are the most important indicators of fertility among semen parameters. Psychological factors: psychological factors such as mental tension of the recipient may affect the regulation of the HPO axis causing ovulation disorders and starting the possibility of conception failure.