TCT is the abbreviation of liquid-based thin-layer cytology test. TCT test is to detect cervical cells and make cytological classification and diagnosis by using liquid-based thin-layer cytology detection system. TCT cervical cancer cytology test has a 100% detection rate of cervical cancer cells and can also detect precancerous lesions, microbial infections such as mycobacteria, trichomonas and chlamydia. Currently, many advanced countries have applied TCT technology for cervical cancer screening in women. Cervical cancer is one of the most prevalent cancers in women worldwide, with approximately 450,000 such cases detected each year. The American Cancer Society estimates that in 1996, approximately 15,700 people in the United States had invasive cancer and 65,000 people had in situ cancer or severe atypical hyperplasia symptoms. Approximately 4,900 deaths occurred as a result. After cervical cancer reaches the invasive stage, treatment requires additional chemotherapy, radiation therapy, or surgery, including hysterectomy. These treatments are very difficult and costly and are not easily successful. Since May 1996, the TCT test technology has gained worldwide recognition. There are more than one thousand users worldwide, in 30 countries and territories. More than two hundred relevant papers have been published in the last two years, with significant improvements in positive test rates, reductions in the number of unsatisfactory samples, and reductions in ASCUS. In TCT testing, the clinician collects a cervical cell sample in the usual way with a TCT-specific sampler, and then instead of applying it directly to the microscopic slide, the collector is placed in a vial with cell preservation solution and rinsed so that a nearly full cell sample is obtained. The patient’s cell sample vial is then sent to the laboratory, where the sample is dispersed and filtered with a fully automated cytometer to reduce the residue of blood, mucus, and inflammatory tissue, thus obtaining a thin layer of well-preserved cells for further microscopic testing and diagnosis. Precautions for TCT sampling: 1. Avoid menstruation as much as possible, no medication 24 hours before sampling, no rinsing, no sexual intercourse, no gynecological examination, use speculum without lubricant, because lubricant will affect the later preparation of the specimen. 2, women in pregnancy in the middle of pregnancy sampling will not affect the pregnancy. 3.Women who receive hormone replacement therapy are as suitable for use. 4.Wipe gently with a cotton swab when there is a lot of secretion, do not use force. 5.If cervical bleeding is evident during sampling, it should be stopped. 6.Avoid repeated sampling within 3 months to avoid false negatives. 7.It is suitable for all women who have or have had sex, basically once every one to two years. for women over 70 years old, if two cytological examinations in the last 5 years are normal, the examination can be stopped.