As we all know, as a window test for kidney disease, urine routine encompasses several screening indicators such as urine protein, red blood cells, white blood cells and urine sugar, which can screen for diseases including nephritis, urinary tract infection, diabetes and interstitial kidney disease. However, routine urinalysis as a testing indicator, for example, when evaluating the effect of kidney disease treatment, is overwhelming, especially when the urine is undetectable after routine urine protein (-), therefore, 24-hour urine protein quantification is particularly important. How to keep a 24-hour urine specimen? Here is an introduction: first of all, choose a point in the early morning (for example, 7:00 am), urinate once at this time, this urine is not wanted; after that, leave all the urine in a container until the next morning at the same point in time to urinate again, this time the urine is wanted; the above is the 24-hour urine, in the first time to urinate is, at the same time in the container to put the appropriate amount of preservative (usually with glutaraldehyde) (Generally, in the opening of the test, the examiner will give preservatives, preservatives are toxic, do not enter!) Do not enter! After keeping the urine, you should measure the total amount (measuring cylinder, measuring cup, if there is no way to measure, you can also take it all to the hospital to measure), and then take some (10-20ml is enough) urine to the hospital for examination. The 24-hour urine protein quantification is very useful for efficacy evaluation. Urine routine examination, negative urine protein corresponds to urine protein quantification below 0.5g, a ++ cannot be fully estimated the amount of urine protein, for example, after a month of treatment, the patient’s urine protein is still ＋＋＋＋, but if the urine protein quantification drops from more than 10g to 4-5g, this indicates that the treatment is still effective; while sometimes the urine protein ＋＋＋＋ drops to ++++, this does not necessarily indicate Especially after the urine protein (routine examination) has turned negative, the urine routine examination can no longer explain the problem, and only through the urine protein quantification can we clarify whether the urine protein has really turned negative and the time needed for treatment, etc.