Gynecological examination of hormone six usually refers to the examination of sex hormone six, namely testosterone, estradiol, progesterone, luteinizing hormone, prolactin and follicle stimulating hormone, mainly to determine whether the patient has ovarian hypofunction and whether there are endocrine disorders, as follows: a. Preparation for the examination: on the 3rd-5th day after the onset of menstruation in women, blood is drawn at 9 am on an empty stomach for a more accurate result. Infertility or amenorrhea, long-term absence of menstruation, can be examined at any time, but fasting is best. The day before the test, you should eat lightly during the day and avoid smoking and alcohol. Fasting from food and water after 10 pm. Stop using sex hormone drugs, such as progesterone, estrogen, etc., one month before the examination. Second, the examination content: 1, testosterone: the normal concentration of blood testosterone in women is 0.7-3.1nmol/L. High blood testosterone value, called hypertestosteronemia, can cause infertility. When suffering from polycystic ovary syndrome, the blood testosterone value will also be increased. The diagnosis should be made according to the clinical manifestations, and other hormones should be measured if necessary. 2. estradiol: the concentration of blood estradiol is 48-521pmol/L in the preovulatory period, 70-1835pmol/L in the ovulatory period, and 272-793pmol/L in the late ovulatory period. Low values are seen in ovarian hypofunction, premature ovarian failure, and Silhan’s syndrome. 3. progesterone: the concentration of blood progesterone is 0-4.8nmol/L in the preovulatory period. Low values of blood progesterone in late ovulation are seen in luteal insufficiency, ovulatory dysfunctional uterine bleeding, etc.; 4. luteinizing hormone: the concentration of blood luteinizing hormone is 2-15mIU/mL in preovulation, 30-100mIU/mL in ovulation and 4-10mIU/mL in late ovulation. The normal value in the non-ovulatory period is 5-25 mIU/mL, and less than 5 mIU/mL indicates gonadotropin insufficiency, which is seen in Schihan’s syndrome. High luteinizing hormone and, if added, high luteinizing hormone, ovarian failure is well established and no additional testing is necessary. Luteinizing hormone/folliculopoietin ≥3 is one of the bases for the diagnosis of polycystic ovary syndrome; 5. prolactin: during the non-lactation period, the normal value of blood prolactin is 0.08-0.92 nmol/L. Higher than 1.0 nmol/L is considered hyperprolactinemia, excess prolactin can inhibit the secretion of folliculopoietin and luteinizing hormone, inhibit ovarian function and ovulation; 6. The concentration of follicle stimulating hormone is 1.5-10mIU/mL in the preovulatory phase, 8-20mIU/mL in the ovulatory phase and 2-10mIU/mL in the late ovulatory phase, and usually 5-40mIU/mL is the normal value. Low values of follicle stimulating hormone are seen during estrogen and progesterone treatment and in Silhan’s syndrome, while high values of follicle stimulating hormone are seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. Analysis of the results: 1. In the six sex hormone tests in women, if the prolactin is high, it is common in hyperprolactinemia or pituitary adenoma; 2. If the proportion of luteinizing hormone is higher than follicle stimulating hormone and is greater than 2, in this case, if there is also an increase in androgen level, the possibility of polycystic ovary syndrome should be considered; 3. If the basal endocrine level of prolactin is high or the level of androgens is high, then hyperprolactinemia or hyperandrogenemia should be considered.