1. Age: >35 years old ovarian reserve capacity declined significantly, but there are individual differences; need to be combined with other indicators; 2. Menstrual cycle: regular shortening of the menstrual cycle often indicates a decline in reserve capacity; 3. Ovarian disease and surgical history of ovarian function damage; 4. Sinusoidal follicle count: the whole ovary count of ≤ 5 or the most cut ≤ 3 suggests a decline in reserve; 5. Basal FSH level: generally ≥ 15 U/L; 6. Basal E2 level: E2 > 80 pg/ml on day 3 of menstruation, regardless of age and FSH level, already clear that the decline in fertility. 15 U/L, which is more significant; 6. Basal E2 level: E2>80pg/ml on the 3rd day of menstruation, regardless of age and FSH level, it is already clear that the fertility has declined. 7. Basal inhibitin and AMH levels: inhibitB<80pg/ml or AMH<8pmol/l, suggesting ovarian hyporesponsiveness; 8. Basal FSH/LH ratio: >3.6 (specificity 95%, sensitivity 85%), suggesting DOR (dysfunction of the ovarian reserve) (FSH can be in the normal range), which is an early manifestation of ovarian dysfunction and often It suggests that the patient responds poorly to superovulation (COH), and the COH regimen and the dose of Gn should be promptly adjusted to improve ovarian responsiveness and obtain the desired pregnancy rate. Because elevated FSH/LH only reflects DOR, not a decline in fertility, once the period of ovulation is obtained, the ideal pregnancy can still be obtained. 9. CC stimulation test: cc100mg, qd, for 5 days on the 5th day of menstruation. If FSH is normal on day 3, normal or mildly elevated on day 10, and E2 rises substantially, ovarian function is suggested to be inadequate. 10. History of previous COH. Diagnostic criteria for ovarian hyporesponsiveness: Most scholars consider that follicles that have reached the mature stage of development, the number of follicles with a diameter of >14mm or the number of eggs retrieved is ≤3-4, and the peak E2 value before ovulation is <300-500pg/ml as ovarian hyporesponsiveness under conventional stimulation of COH regimen. Ovarian hyporesponsiveness was also indicated when one of the following conditions occurred during superovulation: 1) total Gn dosage ≥44; 2) average daily Gn dosage ≥300 U; 3) Gn stimulation cycle ≥15 days. Ovarian hyporesponsiveness is also indicated.