The first test performed by the male partner is semen analysis (some hospitals call it semen routine), which requires 3-5 days without semen discharge, the shortest should be more than 48 hours and the longest not more than 7 days; in the hospital sperm collection room, the patient washes his penis and hands and then masturbates to discharge semen into a sterile wide-mouth glass bottle prepared by the hospital, taking care not to spill it outside and affect the laboratory results; after sperm collection, the specimen is sent to the laboratory in time. If it is difficult to take sperm by yourself, you can ask your wife to assist you, but never use other containers such as condoms to take sperm. For sperm retrieval at home, make sure to send the semen to the laboratory within half an hour, while paying attention to storing the specimen close to the body to ensure the temperature of the semen. Since semen analysis takes more than 1 hour, it is best to visit the clinic 2 hours before the end of the day, not too late. If there is a problem with semen laboratory test, the doctor may selectively perform male examination, prostatitis routine, seminal plasma biochemistry, anti-sperm membrane antibody, sperm function, sperm DNA integrity, scrotal ultrasound, transrectal ultrasound, sex hormone six (some are called endocrine six, note: must be fasting in the morning and have blood drawn before nine o’clock.) The test is conducted to find the cause of the disease so that targeted treatment can be given.