Patients suspected of having prostate cancer routinely undergo rectal examinations, PSA examinations and ultrasound examinations, but what other tests are needed to confirm the diagnosis of prostate cancer? Most patients need to undergo a puncture test to confirm the diagnosis of prostate cancer. The most reliable way to confirm the diagnosis of prostate cancer is by puncture of the prostate gland. Whether the prostate cancer is treated by endocrine therapy or surgery, the final diagnosis is made by puncture. A puncture is performed by using a very long needle to insert 12 or 14 stitches into the prostate through the rectum. It is important to note that since the prostate gland has sensory nerves but no painful nerves in the body, there may be a slight discomfort during the puncture, but it will not feel very strong stinging pain. Therefore, patients should not be overly concerned or fearful.